After YouTube removed Russia Today's German-language channels, the Russian Foreign Ministry warned of retaliation.
The Moscow-backed broadcaster had broken YouTube's COVID-19 disinformation policy, the company said.
The warning is the latest example of Russian officials going to tremendous efforts to impose greater internet control.
YouTube removed the channels on Tuesday, one day after the online video giant stated it will shut down channels that propagate vaccine disinformation.
Russia has recently increased its pressure on global tech companies in order to gain more control over content available to its local audience online.
Roskomnadzor, the Russian media watchdog, demanded that YouTube overturn its decision "immediately."
Roskomnadzor demanded that all limitations be "lifted", in a letter to Google, which controls and owns YouTube.
YouTube issued a warning to RT on Tuesday for breaking its coronavirus misinformation policy, and subsequently shut down two channels for violating user agreements, German media reported.
On Wednesday, Russia's foreign ministry accused YouTube of an "unprecedented act of media aggression" assisted by German authorities, an accusation refuted by Berlin.
Soon after taking office in 2000, President Vladimir Putin tightened his grip on Russia's independent television networks.
However, despite the platform's popularity among Russians under 30 and Putin detractors, Moscow journalist Andrei Loshak told Axios that YouTube has escaped his grasp so far.
"Anyone advocating for such reprisal does not have a good relationship with freedom of the press," Steffen Seibert, Chancellor Angela Merkel's representative, told journalists, adding that YouTube's action "was not a state measure."
Foreign-owned social media outlets have been accused by the Kremlin of meddling in Russian politics, especially by displaying content favorable of imprisoned opposition leader Alexei Navalny.
Roskomnadzor disabled dozens of websites associated to Navalny, whose organizations were outlawed in Russia under "extremism" laws, ahead of parliamentary elections last month.
Later Wednesday, a Moscow court penalized Google 6.5 million rubles ($89,000) for "failing to erase information that Moscow deems unlawful," Reuters disclosed.
Several fines have already been levied against Google for similar offenses. It was also fined for breaking a contentious regulation introduced in 2014 that requires Russian users' personal data to be retained within the country.
